In contrast to the other skin tests, provocation tests are conducted on the organs affected by the allergy. The nasal provocation test is used in cases of hay fever (allergic rhinitis). A solution containing a small amount of the allergen is sprayed into the nose, and the reaction is monitored.

Due to the direct contact with the allergen, the reaction is in some cases more severe than predicted. Sometimes a life-threaning anaphylactic shock can result. As a result, it is important that the test be overseen by or performed by a doctor with specific allergy expertise.